HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis^® Express RP-Amide ("Green" Mobile Phase Conditions)

column

Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)

flow rate

0.4 mL/min

pressure

138 bar (2000 psi) column, 248 bar (3600 psi) total

column temp.

55 °C

detector

UV, 215 nm

injection

1 μL

sample

50 μg/mL in mobile phase