HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase
Materials
분석 컬럼
제품 번호
설명
가격
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 5 cm × 4.6 mm표준
제품 번호
설명
가격
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Alprazolam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Clonazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase
[A] water; [B] acetonitrile; (62:38, A:B)
flow rate
1.4 mL/min
pressure
2600 psi (179 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
0.1 g/mL of each in 20% methanol
설명
분석 메모
The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany