HPLC Analysis of Amyloid β-Protein Fragment 10-35 on Discovery^® BIO Wide Pore C18

column

Discovery BIO Wide Pore C18, 25 cm × 4.6 mm I.D., 5 μm particles (568223-U)

mobile phase

[A] 0.1% TFA in water; [B] 0.1% TFA in water:0.1% TFA in acetonitrile; (25:75)

flow rate

1.3 mL/min

column temp.

30 °C

detector

UV, 215 nm

injection

10 μL, 1 mg/mL in water