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Merck

GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel QuE Z-Sep+, Fast GC Analysis

GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis application for SPE, suitable for GC

Materials

SPE 튜브 또는 플레이트

제품 번호
설명
가격

Supel QuE

Acetate Tube, pk of 50, AOAC® 2007.01

CONDITIONS

sample preparation

Dispersive (QuEChERS)

sample/matrix

Avocado spiked with pesticides at 20 ng/g

extraction tube

Supel QuE Acetate extraction tube (55234-U)

extraction process

add 25 mL of acetonitrile; shake for 1 minute; add contents of Supel QuE acetate extraction tube; shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes

clean-up tube

Supel Que Z-Sep+ cleanup tube (55296-U)

clean-up process

transfer 3 mL of the acetonitrile layer into a Supel Que Z-Sep+ cleanup tube; shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis

column

SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)

oven

70 °C (2 min), 15 °C/min to 325 °C (6 min)

inj. temp.

60 °C (0.28 min), 600 °C/min to 325 °C (5 min)

detector

MSD

MSD interface

330 °C

carrier gas

helium, 1 mL/min, constant

injection

PTV solvent vent; 10 μL, injection dispense speed 15000 μL/min

liner

4 mm I.D. FocusLiner with taper

sample

pesticides spiked at 20 ng/g

설명

분석 메모

About this analysis: This application demonstrates the use of dispersive SPE for the extraction of pesticides from Avocado. Cleanup is achieved using Z-Sep + for fat removal. FInal analysis of the extracts was done by LVI-GC/MS SIM on an SLB-5ms column.

법적 정보

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC