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Merck

GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLBยฎ-5ms (20 m x 0.18 mm I.D., 0.18 ฮผm) after QuEChERS Cleanup using Supelโ„ข QuE Z-Sep, Fast GC Analysis

GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis suitable for GC, application for SPE

Materials

SPE ํŠœ๋ธŒ ๋˜๋Š” ํ”Œ๋ ˆ์ดํŠธ

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Supelโ„ข QuE

Empty Centrifuge Tube with Lid, centrifuge tube volume 50 mL, pk of 50, suitable for EN 15662:2008 per BS

Supelโ„ข QuE

Non-Buffered Tube 1, pk of 50

Supelโ„ข QuE

Z-Sep+ Bulk, pkg of 20 g

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SLBยฎ-5ms Capillary GC Column

L ร— I.D. 20 m ร— 0.18 mm, df 0.18 ฮผm

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PCB standard solution 7

analytical standard, 10 μg/mL in isooctane

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

10 g whole cow′s milk

extraction tube

Supelโ„ข QuE Non-buffered tube (4 g magnesium sulfate, 1 g sodium chloride) (55294-U)

clean-up tube

Supelโ„ข QuE Z-Sep 150 mg, magnesium sulfate (150 mg, part no. 63135) (55299-U)

column

SLB-5ms, 20 m x 0.18 mm I.D., 0.18 μm (28564-U)

oven

75 ยฐC (1 min), 12 ยฐC/min to 340 ยฐC (10 min)

inj. temp.

250 ยฐC

detector

μ-ECD, 340 ยฐC

carrier gas

hydrogen, 1.2 mL/min, constant

injection

1 μL splitless (0.75 min)

liner

4 mm I.D. FocusLinerโ„ข with taper

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Reference: Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034

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This application demonstrates the use of dispersive SPE or "QuEChERS" for the extraction and cleanup of whole cowโ€ฒs milk for GC-ECD analysis of polychlorinated biphenyl (PCBs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >70% for all PCB congeners evaluated except no. 209, which was recovered at 68%.
Extraction solvent: acetone:hexane (1:1), 20 mL
Clean-up volume: 1 mL

๋ฒ•์  ์ •๋ณด

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC