HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentisยฎ Express Biphenyl with UV Detection after Extraction
Materials
๊ฐ์๋ ์ปฌ๋ผ
Ascentisยฎ Express Biphenyl Guard Column
pkg of 3 ea, 2.7 μm particle size, L ร I.D. 0.5 cm ร 2.1 mm๋ถ์๋ฌผ
Supelโข QuE
Empty Centrifuge Tube with Lid, centrifuge tube volume 50 mL, pk of 50, suitable for EN 15662:2008 per BSCertified Vial Kit, Low Adsorption (LA), 2 mL, pk of 100
volume 2 mL, amber glass vial (with marking spot), natural PTFE/silicone septa, thread for 9 mm๋ถ์ ์ปฌ๋ผ
CONDITIONS
extraction tube
SupelQue Non-buffered tube 2 (55295-U)
extraction process
Transfer the solution into 50 mL plastic tube (55248-U); add 10 mL acetonitrile; shake for 1 minute by hand; add contents of the Supel QuE Non-Buffered Extraction Tube 2 (55295-U); (shake for 5 minutes using QuEChERS shaker (55278-U); centrifuge for 5 minutes at 5000 rpm; collect top acetonitrile layer; no cleanup is required.)
column
Ascentis Express Biphenyl, 10cm x 2.1mm, 2.7 μm particles (64065-U)
mobile phase
(A) 0.1% TFA in water; (B) 0.1% TFA in acetonitrile
gradient
47% B to 50% B in 13 min.; 100% B in 0.1 min; held at 100% B for 3 min; 47% B in 0.1 min; held at 47% B for 2.5 min.
flow rate
0.7 mL/min
pressure
5000 psi (345 bar)
column temp.
35 ยฐC
detector
UV, 220 nm
injection
5 μL
์ค๋ช
๋ถ์ ๋ฉ๋ชจ
dissolve in heated water, 1 gummy candy in 20 mL
๋ฒ์ ์ ๋ณด
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany