An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay.

An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0-3.0μgkg