콘텐츠로 건너뛰기
Merck
  • Synthesis of a carboxamide linked T*T dimer and its incorporation in oligonucleotides.

Synthesis of a carboxamide linked T*T dimer and its incorporation in oligonucleotides.

Nucleic acids research (1993-11-11)
A Chur, B Holst, O Dahl, P Valentin-Hansen, E B Pedersen
초록

The condensation of 5'-O-protected 3'-O-(2-aminoethyl)thymidine with 1,2-dideoxy-1-thyminyl-beta-D-erythro-pentofuranuronic acid gives a T*T dimer with * representing a 3'-OCH2CH2NHC(O)-4' linkage connecting the two pentofuranosyl moieties. The incorporation of this dimer in oligonucleotide sequences show only moderately lowered Tm values when hybridized with a complementary DNA relative to the unmodified DNA duplex. Consistently, no looped-out or bubble-type structure could be detected in DNA duplexes with an internal T*T module. Moreover, the 5-atom carboxamide linker causes complete stop on DNA polymerization and on exonuclease III degradation.

MATERIALS
제품 번호
브랜드
제품 설명

Sigma-Aldrich
2-Chloroethylamine hydrochloride, 99%