- Docking phospholipase A2 on membranes using electrostatic potential-modulated spin relaxation magnetic resonance.
Docking phospholipase A2 on membranes using electrostatic potential-modulated spin relaxation magnetic resonance.
Science (New York, N.Y.) (1998-04-16)
Y Lin, R Nielsen, D Murray, W L Hubbell, C Mailer, B H Robinson, M H Gelb
PMID9506941
초록
A method involving electron paramagnetic resonance spectroscopy of a site-selectively spin-labeled peripheral membrane protein in the presence and absence of membranes and of a water-soluble spin relaxant (chromium oxalate) has been developed to determine how bee venom phospholipase A2 sits on the membrane. Theory based on the Poisson-Boltzmann equation shows that the rate of spin relaxation of a protein-bound nitroxide by a membrane-impermeant spin relaxant depends on the distance (up to tens of angstroms) from the spin probe to the membrane. The measurements define the interfacial binding surface of this secreted phospholipase A2.
MATERIALS