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Merck
  • Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue.

Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue.

Free radical biology & medicine (2000-01-21)
E Katsanidis, P B Addis
초록

Tocopherols and tocotrienols are being increasingly recognized to have an important role in the prevention of atherosclerosis. It has been reported that they protect low-density lipoprotein (LDL) and tissues from oxidative stress and that tocotrienols can reduce plasma cholesterol levels. Two isocratic high-performance liquid chromatography (HPLC) methods for simultaneous analysis of tocopherols, tocotrienols, and cholesterol in muscle tissue were developed. Method A involves basic saponification of the sample, but causes losses of the gamma- and delta-homologs of vitamin E. Method B does not involve saponification, thereby protecting the more sensitive homologs. Both permit rapid analysis of multiple samples and neither requires specialized equipment. These methods may provide techniques useful in simultaneous assessment of oxidative stress status (OSS) and cholesterol levels.

MATERIALS
제품 번호
브랜드
제품 설명

Supelco
β-Tocotrienol, analytical standard