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Merck

Free-metal ion depletion by "Good's" buffers.

Science (New York, N.Y.) (1983-08-19)
R Nakon, C R Krishnamoorthy
초록

Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine). The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES). Bicine and TES buffer not only hydrogen ions but also metal ions. The metal complexes of "Good's" buffers also buffer hydrogen ions by secondary reactions. The consequences of these reactions are considered in relation to biomedical research.

MATERIALS
제품 번호
브랜드
제품 설명

Sigma-Aldrich
TES sodium salt, ≥99% (titration)
Sigma-Aldrich
BICINE, BioXtra, ≥99% (titration)
Sigma-Aldrich
BICINE, ≥99% (titration)