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Merck

Thiol-sensitive promoters of Escherichia coli.

Journal of bacteriology (1988-07-01)
G T Javor, C D Stringer, J Ryu
초록

Mu dX(lac) insertion mutants of Escherichia coli CSH50 in which the expression of the lacZ gene was sensitive to the presence of exogenous 1-thioglycerol or dithiothreitol were isolated. Both stimulatory and inhibitory mutants were found. The existence of several thiol-sensitive promoters suggests that exogenous thiols may provoke global stress responses in E. coli.

MATERIALS
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Sigma-Aldrich
1-Thioglycerol, BioXtra, ≥97%