기술
HPLC: suitable
검사 파라미터
column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B)
flow rate: 1 mL/min
pressure: 2200 psi (152 bar)
column temp.: 30 °C
detector: UV, 250 nm
injection: 5 μL
sample: 50 μg/mL in 75:25, water:acetonitrile
적합성
application for HPLC
응용 분야
pharmaceutical (small molecule)
분석 메모
Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
분석물
설명
Acetylsalicylic acid, analytical standard
Salicylic acid, BioXtra, ≥99.0%
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