생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
2C14, recombinant monoclonal
설명
recombinant, expressed in HEK 293 cells
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt 41.25 kDa
observed mol wt ~41 kDa
정제법
using Protein A
종 반응성
human
종 반응성(상동성에 의해 예측)
porcine, feline, canine, monkey
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG
에피토프 서열
Internal
단백질 ID 수납 번호
UniProt 수납 번호
환경친화적 대안 카테고리
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... ATXN3(4287)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
특이성
Clone 2C14 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Ataxin-3. It targets an epitope within 20 amino acids from the internal region.
면역원
His-tagged recombinant fragment corresponding to 138 amino acids from the internal region of human Ataxin-3.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ataxin-3 in MCF-7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ataxin-3 in SH-SY5Y cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Ataxin-3 recombinant fragment with a KD of 9.5 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ataxin-3 in human testis and human urinary bladder tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ataxin-3 in MCF-7 cell lysate.
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ataxin-3 in MCF-7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ataxin-3 in SH-SY5Y cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Ataxin-3 recombinant fragment with a KD of 9.5 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ataxin-3 in human testis and human urinary bladder tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ataxin-3 in MCF-7 cell lysate.
표적 설명
Ataxin-3 (UniProt: P54252; also known as EC:3.4.19.12, Machado-Joseph disease protein 1, Spinocerebellar ataxia type 3 protein, ATXN3) is encoded by the ATXN3 (also known as ATX3, MJD, MJD1, SCA3) gene (Gene ID: 4287) in human. Ataxin-3 is a ubiquitously expressed nuclear protein that serves as a deubiquitinating enzyme (DUB) involved in protein homeostasis and quality control, myogenesis and degradation of misfolded chaperone substrates. As a DUB, it has two distinct features. Its N-terminal Josephin domain confers cysteine protease activity to Ataxin-3 which is important for hydrolyzing ubiquitin (Ub) linkages. The second important feature is its three Ub-interacting motifs (UIMs) through which it binds Ub conjugates and ubiquitinated proteins and bring them into proximity to trim or edit specific linkages within these Ub conjugates. It binds long poly-Ub chains and trims them. However, it has weak or no activity against chains of 4 or less Ub. Ataxin-3 is also involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP (C- terminus of HSC70-Interacting Protein). It is recruited to monoubiquitinated STUB1/CHIP and restricts the length of Ub chain attached to STUB1/CHIP substrates and prevents further chain extension. It is reported to regulate autophagy via deubiquitination of lysine 402 of Beclin 1. Mutations in ATXN3 gene are known to cause Spinocerebellar ataxia 3 (SCA3), an autosomal disorder that is manifested in progressive incoordination of gait and poor coordination of hands, speech, and eye movements. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Durcan, DM., and Fon, EA. (2013). Front. Neurol. 4; 46; Tzvetkov, N., and Breuer, P. (2007). Biol. Chem. 388(9); 973-978; Mao, Y., et al. (2005). Proc. Natl. Acad. Sci. USA. 102(36); 12700-127005).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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