Y2377

2x YT medium

Powder microbial growth medium

• 동의어(들): 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
• UNSPSC 코드: 41106225
• NACRES: NA.85

속성

• Grade: for molecular biology
• Quality Level: 200
• 무균: non-sterile
• 형태: powder
• 기술: microbiological culture: suitable
• pH: 6.8-7.2(3.1% solution)
• 응용 분야: food and beverages; microbiology
• 저장 온도: room temp
• 적합성: nonselective for Escherichia coli; nonselective for coliforms

설명

일반 설명

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

애플리케이션

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

특징 및 장점

2xYT powder provides:

• Standard formulation
• An inexpensive alternative to liquid

성분

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

제조 메모

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

재구성

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)