T9069

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

• 동의어(들): DNA Topoisomerase I
• 효소 위원회 번호: 5.99.1.2 (BRENDA, IUBMB)
• MDL number: MFCD03458765
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in baculovirus
• Quality Level: 200
• 형태: buffered aqueous glycerol solution
• 분자량: 100 kDa
• 농도: ≥2 units/μL
• UniProt 수납 번호: P11387
• 저장 온도: −70°C
• 유전자 정보: human ... TOP1(7150)

설명

일반 설명

Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.

애플리케이션

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.

생화학적/생리학적 작용

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

단위 정의

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

물리적 형태

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H360D
• 예방조치 성명서: P201 - P308 + P313
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable