T1299

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse

clone TH-2, ascites fluid

• 동의어(들): Anti-Tyrosine Hydroxylase, Mouse Anti-Tyrosine Hydroxylase, Tyrosine Hydroxylase Detection
• MDL number: MFCD00213051
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: TH-2, monoclonal
• 포함: 15 mM sodium azide
• 종 반응성: human, monkey, guinea pig, rat, sheep, bovine, rabbit
• 기술: immunohistochemistry: suitable; immunoprecipitation (IP): suitable using native or SDS-denatured TH.; western blot: 1:10,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
• 동형: IgG1
• UniProt 수납 번호: P07101
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TH(7054) ; rat ... Th(25085)

설명

일반 설명

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tyrosine hydroxylase (TH) RNA undergoes alternative splicing resulting in four isoforms of TH subunit protein in humans. The four human isoforms are TH-1 (55.6 kDa), TH-2 (56 kDa), TH-3 (58.1 kDa), and TH-4 (58.5 kDa). Only humans express all four isoforms.

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O₂ as cosubstrates, and Fe²⁺ as a cofactor.

특이성

The antibody recognizes an epitope present in the N-terminal region (approx. aa 9-16) of both rodent and human tyrosine hydroxylase. Clone TH-2 reacts with the intact TH subunits.

면역원

rat tyrosine hydroxylase (TH).

애플리케이션

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in

• western blotting
• indirect immunofluorescence
• immunostaining
• immunohistochemistry
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunoprecipitation

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in western blotting and immunocytochemistry.

생화학적/생리학적 작용

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable