SAB4200107

Anti-CHD4 antibody, Mouse monoclonal

clone 3F2/4, purified from hybridoma cell culture

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: 3F2/4, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~250 kDa
• 종 반응성: human, mouse
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• 동형: IgG2a
• UniProt 수납 번호: Q14839
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CHD4(1108)

설명

일반 설명

Monoclonal Anti-CHD4 (mouse IgG2a isotype) is derived from the hybridoma 3F2/4 produced by the fusion of mouse myeloma cells and splenocytes from CD-1 mice immunized with a synthetic peptide derived from human chromodomain helicase DNA binding protein 4 (CHD4) conjugated to keyhole limpet hemocyanine (KLH). CHD4 is one of the subunits of Mi-2, an ATP-dependent helicase. It belongs to the sucrose non-fermentable (SNF2)/Rad54 helicase family of proteins. The CHD4 gene is mapped to human chromosome 12p13.31 and is highly conserved among animals and plants.

면역원

synthetic peptide derived from human CHD4, conjugated to KLH

애플리케이션

Monoclonal Anti-CHD4 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

생화학적/생리학적 작용

Chromodomain helicase DNA binding protein 4 (CHD4) interacts with metastasis-associated protein (MTA1/2), histone deacetylase 1 (HDAC1) and RbAp46 in the nucleosome remodelling and deacetylation (NuRD) complex. Interestingly, antibodies against CHD4 have been found to be involved in the onset of dermatomyositis (DM), an autoimmune disease which is a form of idiopathic inflammatory myopathy (IIM). Variation in the gene due to mutation is observed in endometrial cancer. CHD4 regulates homologous recombination and thus maintains genome stability.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable