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일반 설명
Phosphothreonine-BSA is a Phosphothreonine antibody inhibitor which blocks the reactivity of anti-phosphothreonine antibody. Antibodies against phosphorylated amino acid can be used as a tool for identification, quantification and immunoaffinity isolation of activated cellular proteins. The product specifically blocks the reactivity of anti-phosphothreonine antibody but does not block anti-phosphoserine- or anti-phosphotyrosine specific antibodies.
특이성
Working dilution is at least 10 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphothreonine (Sigma Product No. P 3555) in ELISA.
애플리케이션
Phosphothreonine-BSA can be used in various immunochemical assays like ELISA and immunoblotting for the specific inhibition of the reactivity of anti-phosphothreonine antibodies.
Phosphothreonine-BSA has been used in Pro-Q Diamond phosphoprotein gel staining method to detect phosphoamino acids.
생화학적/생리학적 작용
O-phospho-L-threonine conjugated to bovine serum albumin. Phosphothreonine antibody inhibitor specifically blocks the reactivity of anti-phosphothreonine antibody. It does not block the activity of anti-phosphotyrosine or anti-phosphoserine-specific antibodies.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry
Schulenberg B, et al.
Electrophoresis, 25(15), 2526-2532 (2004)
Matthew G Vander Heiden et al.
Science (New York, N.Y.), 329(5998), 1492-1499 (2010-09-18)
Proliferating cells, including cancer cells, require altered metabolism to efficiently incorporate nutrients such as glucose into biomass. The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucose by aerobic glycolysis and contributes to anabolic metabolism. Paradoxically, decreased pyruvate
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