추천 제품
사용
sufficient for 100 colorimetric or fluorometric tests
검출 방법
colorimetric
fluorometric
관련 질환(들)
endocrinological disorders, diabetes; neurological disorders
저장 온도
−20°C
관련 카테고리
일반 설명
Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.
애플리케이션
Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.
적합성
The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.
원리
Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Influence of ketamine on amino acid neurotransmitters secretion by nerve cells in vitro.
Shi M, et al.
Bangladesh Journal of Pharmacology, 11(S1), 55-60 (2016)
The Impact of Inflammation on Pancreatic β-Cell Metabolism, Function and Failure in T1DM and T2DM: Commonalities and Differences.
Newsholme P, et al.
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Essentials of medical biochemistry: with clinical cases.
Ha C E and Bhagavan N V
ESSENTIALS OF MEDICINAL CHEMISTRY, 2011 null
Role of the Malate-Aspartate Shuttle on the Metabolic Response to Myocardial Ischemia
Ming Lu, et al.
Journal of Theoretical Biology, 254(2), 466-475 (2008)
Philip H Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(35), E7226-E7235 (2017-08-16)
Cyclic di-3',5'-adenosine monophosphate (c-di-AMP) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. Our earlier studies showed that c-di-AMP regulates central metabolism in Listeria monocytogenes by inhibiting its pyruvate carboxylase (LmPC)
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