material
polycarbonate housing
polyethersulfone membrane
무균
non-sterile
포장
pack of 25 pieces
제조업체/상표
Cytiva 28-9322-25
재고 정보
not available in Japan
튜브 크기
~2.2 mL
작업 용적
500 μL
공극 크기
10 kDa MWCO
저장 온도
room temp
일반 설명
Designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest.
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest.
특징 및 장점
- Compatible pH range is from pH 1 to 9.
- Patented dead-stop technology, which ensures that samples cannot be concentrated to dryness and enables direct concentrate recovery
- One-step sample concentration in a single tube for minimal sample handling and reduced sample loss.
- Vertical polyethersulfone membrane minimizes membrane blockage and tolerates high flow rates.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Vivaspin is a registered trademark of Sartorius Stedim Biotech GmbH
Storage Class Code
13 - Non Combustible Solids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Susanna Raho et al.
Nature metabolism, 2(12), 1373-1381 (2020-11-25)
The oncogenic KRAS mutation has a critical role in the initiation of human pancreatic ductal adenocarcinoma (PDAC) since it rewires glutamine metabolism to increase reduced nicotinamide adenine dinucleotide phosphate (NADPH) production, balancing cellular redox homeostasis with macromolecular synthesis1,2. Mitochondrial glutamine-derived
문서
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프로토콜
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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