IPI-2I

93100622, Porcine intestine, Epithelial-like

• UNSPSC 코드: 41106514

속성

• product name: IPI-2I, 93100622
• 생물학적 소스: Porcine intestine
• 성장 모드: Adherent
• 핵형: Heteroploid
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Boar Miniature male ileum, SV40 transformed

세포주 설명

Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.

애플리케이션

Studies of epithelial cell differentiation.

배양 배지

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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