C6 Cell Line from rat

92090409, rat nerve (glial tumor), Neuronal

• 동의어(들): C-6 Cells, RGC-6 Cells
• UNSPSC 코드: 41106514

속성

• product name: C6 Cell Line from rat, 92090409
• 생물학적 소스: rat nerve (glial tumor)
• 설명: Rat glial tumor
• 성장 모드: Adherent
• 핵형: 2n = 42
• 형태학: Neuronal
• 제품: S-100 protein, somatotrophin
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat glial tumour

세포주 설명

Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates and has been found in brain tumours. Synthesis of S-100 increases 10 fold as cells reach confluency.

애플리케이션

C6 cell line has been used to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions. It has also been used to study the cytotoxic and antioxidant properties of supercritical CO₂ extract (SCE) of old man′s beard (Usnea barbata).

C6 cells are reported to produce elevated levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids

배양 배지

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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