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Merck
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Key Documents

C-20216

Sigma-Aldrich

Keratinocyte Growth Medium 2

Basal Medium, phenol red-free, 500 ml

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

형태

aqueous solution

포장

pkg of 500 ml

기술

cell culture | mammalian: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Keratinocyte Basal Medium 2, phenol red-free (supplements sold separately)

애플리케이션

Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells. The medium is optimized for primary human cells but can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.Recommended for:Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskinNormal Human Epidermal Keratinocytes (NHEK), adult donor

성분

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

품질

All lots of PromoCell Keratinocyte Growth Medium 2 are subjected to comprehensive quality control tests using primary human keratinocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested keratinocytes. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

저장 및 안정성

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

계대배양 정규 작업

Click here for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서

Primary human keratinocytes and serum-free keratinocyte growth media for the feeder cell-free in vitro cultivation of epidermal keratinocytes. Protocols for handling, cell passaging, media, and product use.

Primary human keratinocytes and serum-free keratinocyte growth media for the feeder cell-free in vitro cultivation of epidermal keratinocytes. Protocols for handling, cell passaging, media, and product use.

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