C-12295

Human Uterine Microvascular Endothelial Cells (HUtMEC)

500,000 cryopreserved cells

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human uterus (myometrium)
• 포장: pkg of 500,000 cells
• 형태학: ( endothelial)
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

일반 설명

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세포주 기원

Uterus

애플리케이션

Primary Human Uterine Microvascular Endothelial Cells (HUtMEC) are isolated from the myometrium (middle layer of the uterine wall) from a single donor who is not pre-treated with hormones. The cells are provided in a cryopreserved format. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin. During pregnancy HUtMEC propagate intensely and change their phenotype and expression pattern in response to sex steroids. This includes up-regulation of HUtMEC nitric oxide synthase activity, modulation of adhesion molecule expression, and an increase in endothelial cell survival and angiogenic activity. HUtMEC are involved in medical conditions like uterine fibroma or adenomysis.

품질

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

경고

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

계대배양 정규 작업

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기타 정보

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

추천 제품

Recommended Primary Cell Culture Media:Link

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable