MABT379

Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1

clone 5F8.1, from mouse

• 동의어(들): Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 5F8.1, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_060513
• UniProt 수납 번호: Q5XXA6
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ANO1(55107)

설명

일반 설명

Discovered on gastrointestinal stromal tumors protein 1 (Dog1), also known as Anoctamin-1/TMEM16A, is a novel multi-pass membrane protein belonging to the anoctamin family. Anoctamin-1/TMEM16A functions as a Ca2+-activated Cl- channel and is a required component of tracheal development. Expression of Anoctamin-1/TMEM16A is elevated in the gastrointestinal stromal tumors (GISTs), KIT signaling-driven mesenchymal tumors of the GI tract. It is also expressed at more moderate levels in epithelia from foregut, kidney, lung, pancreas and salivary glands. Recent studies suggest that it may serve a roll as a diagnostic marker in the classification of GISTs.

특이성

This antibody recognizes isoforms 1 and 2 of Anoctamin-1 based upon immunogen design.

면역원

Epitope: N-terminal cytoplasmic domain

GST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human Anoctamin-1/TMEM16A.

애플리케이션

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Anoctamin-1/TMEM16A in human prostate cancer sections.

This Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.

품질

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.

표적 설명

~114 kDa and ~98 kDa observed. Three isoforms are known to exist at 114 kDa (isoform 1), 98 kDa (isoform 2), and 74 kDa (isoform 3).

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable