추천 제품
생물학적 소스
mouse
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
TRA-2-10, monoclonal
종 반응성
human
포장
antibody small pack of 25 μL
기술
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
human ... CD46(4179)
관련 카테고리
일반 설명
Membrane cofactor protein (UniProt: P15529; also known as TLX, Trophoblast leukocyte common antigen, CD46) is encoded by the CD46 (also known as MCP; MIC10) gene (Gene ID: 4179) in human. CD46 is a single-pass type I membrane protein that is expressed by all cells except erythrocytes. It acts as a cofactor for complement factor I, a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. CD46 is synthesized with a signal peptide (aa 1-34), which is cleaved off in the mature form. The mature form contains an extracellular domain (aa 35-343), a transmembrane domain (aa 344-366), and a cytoplasmic domain (aa 367-392). It also contains four Sushi domains where Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles Virus H protein, domains 2 and 3 are essential for Herpes virus 6 binding, and domains 3 and 4 are required for interaction with Streptococcus Pyrogens M protein and are the most important for interaction with C3b and C4b. CD46 can be glycosylated on Asn 83, 114, and 273 and N-glycosylation on Asn 114 and 273 are required for its cytoprotective function. N-glycosylation on Asn 114 is also required for Measles virus binding and N-glycosylation on Asn-273 is required for Neisseria binding. CD46 can also be extensively O-glycosylated in the Ser/Thr-rich domain, which is required for Neisseria binding, but not for Measles virus binding. Mutations in CD46 gene have been linked to hemolytic uremic syndrome atypical 2 that has been characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal failure.
특이성
Clone TRA-2-10 specifically detects CD46 in human cells.
면역원
2102Ep human embryonal carcinoma cells.
애플리케이션
Anti-CD46, clone TRA-2-10, Cat. No. MABF2182, is a mouse monoclonal antibody that detects Membrane cofactor protein (CD46) and has been for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Inhibition assay.
Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).
Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).
Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Research Category
Inflammation & Immunology
Inflammation & Immunology
품질
Evaluated by Flow Cytometry in HeLa cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD46 in one million HeLa cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD46 in one million HeLa cells.
표적 설명
43.75 kDa calculated.
물리적 형태
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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