MABD129

Anti-PRDM1 Antibody, clone 5E7

ascites fluid, clone 5E7, from mouse

• 동의어(들): PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 5E7, monoclonal
• 종 반응성: human
• 기술: western blot: suitable
• 동형: IgG1
• UniProt 수납 번호: O75626
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PRDM1(639)

설명

일반 설명

PRDM1/Blimp1 is a repressor of beta-interferon gene expression. In the nucleus, PRDM1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, PRDM1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade. The transcription of this gene is increased upon virus infection.

면역원

Purified recombinant fragment of human PRDM1 expressed in E. Coli.

애플리케이션

Detect PRDM1 using this mouse monoclonal antibody, Anti-PRDM1 Antibody & clone 5E7 validated for use in western blotting.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

품질

Evaluated by Western Blotting in Raji, L1210, and TPH-1 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PRDM1 in Raji, L1210, and TPH-1 cell lysates.

Optimal working dilutions must be determined by end user.

표적 설명

~88 kDa observed. Uncharacterized bands may appear in some lysate(s).

물리적 형태

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Raji, L1210, and TPH-1 cell lysates

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable