MAB1961H-I

Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate

clone P1F6, from mouse, PE

• 동의어(들): Integrin alpha-V, CD51, Integrin alpha V, Vitronectin receptor subunit alpha, Integrin beta-5
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: PE
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: P1F6, monoclonal
• 종 반응성: human, hamster, chicken
• 기술: flow cytometry: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002210.4; NM_002213.4
• UniProt 수납 번호: P06756; P18084
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGAV(3685)

설명

일반 설명

Integrin alpha-V (UniProt P06756; CD51, Integrin alpha V, Vitronectin receptor subunit alpha) and Integrin beta-5 (UniProt P18084) are encoded by the ITGAV (MSK8, VNRA, VTNR; Gene ID 3685) and the ITGB5 (Gene ID 3693) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit associated in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence present in the extracellular matrix components, such as fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaVbeta5 is the primary mechanotransducer that mediates lung myofibroblast contraction-induced latent TGF-beta1 activation. Increased alphaVbeta5 expression is reported to be associated with myofibroblast differentiation. Integrin alphaVbeta5 is shown to interact with Thy-1 in an RLD-dependent manner. Thy-1 expression prevents fibroblast contraction-induced latent TGF-beta1 activation and lung myofibroblast differentiation.

특이성

Clone P1F6 recognizes αVβ5 without cross-reactivity toward αVβ3 (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948).

면역원

Epitope: Extracellular region.

Human UCLA P3 lung carcinoma cells (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948).

애플리케이션

Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate is an antibody against Integrin αVβ5 for use in Flow Cytometry.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

The unconjugated antibody (Cat. No. MAB1961 & MAB1961Z) is shown to be suitable also for Flow cytometry, Functional blocking, Immunocytochemistry, Immunohistochemistry, and Immunoprecipitation applications.

품질

Evaluated by Flow Cytometry in HT1080 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Integrin αVβ5 in HT1080 cells.

표적 설명

112.7 kDa (alpha-V) and 86.34 kDa (beta-5) calculated. ~150 kDa (alpha-V) and 95 kDa (beta-5) reported (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948) due to glycosylation.

물리적 형태

Protein A purified

Purified mouse monoclonal IgG1 antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable