MAB1864-I

Anti-alpha-Tubulin Antibody, tyrosinated, clone YL1/2

clone YL 1/2, from rat

• 동의어(들): Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, alpha-Tubulin, tyrosinated
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: YL 1/2, monoclonal
• 종 반응성: yeast, porcine, rat, chicken, bovine, human, mouse
• 기술: ELISA: suitable; electron microscopy: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; radioimmunoassay: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_013625
• UniProt 수납 번호: Q71U36
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TUBA1A(7846)

설명

일반 설명

Tubulin alpha-1 chain (UniProt P09733; also known as alpha-tubulin) is encoded by the TUB1 gene (Gene ID 854889) in yeast. Microtubules are dynamic protein filaments involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Microtubules are assembled from α- and β-tubulin subunits, both of which are subjected to posttranslational modifications, including the removal of the C-terminus aromatic amino acid from α-tubulin (tyrosine in mammals and phenylalanine in yeast) by carboxypeptidase actifvity. Because the C-terminus tyrosine/phenylalanine is preceded by glutamate (E) residues, the detyrosinated/dephenylalaninated α-tubulin is also referred to as Glu-tubulin. While tyrosine can be reattached to the C-terminus of the Glu-tubulin by tubulin-tyrosine ligase in mammals, phenylalanine is not added posttranslationally to the Glu-tubulin in yeast despite the presence of the yeast YBR094w gene that exhibits significant homology to tubulin-tyrosine ligase in other organisms.

특이성

Expected to react with a broad range of species based on 100% sequence homology.

Reacts with alpha-tubulin with phenylalanine (yeast) or tyrosine (mammalian and chicken) at the C-terminus (PMID 6204858). Does not react toward posttranslationally modified alpha-tubulin lacking the C-terminus Phe or Tyr.

면역원

Epitope: C-terminus

Purified yeast tubulin

애플리케이션

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-alpha-Tubulin Antibody, tyrosinated, clone YL1/2 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry, Electron Microscopy, ELISA and Radioimmunoassay for the detection of alpha-Tubulin.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected tyrosinated alpha-tubulin in 10 µg of C2C12 cell lysate.
Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected tyrosinated alpha-tubulin in A431, HeLa and HUVEC cells.
Immunocytochemistry Analysis: Representative lots detected phenylalaninated alpha-tubulin immunoreactivity in yeast and tyrosinated alpha-tubulin immunoreactivity in HEK293 cells (Kilmartin J.V., et al. (1982). J Cell Biol. 93(3):576-582; Bandyopadhyay, B., et al. (2007). J Biol Chem. 282(22):16454-16464).
Immunohistochemistry Analysis: A representative lot detected microtubules tyrosinated alpha-tubulin immunoreactivity in mid-streak level section of Hamburger-Hamilton stage 4 (HH4) chick embryos (Nakaya, Y., et al. (2008). Nat. Cell Biol. 10(7):765-775).
Electron Microscopy Analysis: Representative lots detected microtubules phenylalaninated alpha-tubulin immunoreactivity using crude yeast nuclear envelope preparations and microtubules tyrosinated alpha-tubulin immunoreactivity in NIH/3T3 cells (Wehland, J., and Willingham, M.C. (1983). J Cell Biol. 97(5 Pt 1):1476-1490; Kilmartin J.V., et al. (1982). J Cell Biol. 93(3):576-582).
ELISA Analysis: The antigenicity of clone YL1/2 was determined by competitive ELISA using pig brain-derived tubulin against alpha-tubulin peptides with or without Tyr/Phe at the C-terminal end (Wehland, J., et al. (1984). EMBO J. 3(6):1295-1300).
Radioimmunoassay Analysis: The immunoreactivity of clone YL1/2 toward chick brain tubulin and yeast tubulin was determined by radioimmunoassays (Kilmartin J.V., et al. (1982). J Cell Biol. 93(3):576-582).

품질

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected tyrosinated alpha-tubulin in 10 µg of A431 cell lysate.

표적 설명

~55 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable