CKT2MAG-97K

MILLIPLEX^® Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)

• UNSPSC 코드: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

속성

• Quality Level: 200
• 종 반응성: canine
• 제조업체/상표: Milliplex^®
• assay range: accuracy: 96-102%; sensitivity: 0.016-0.026 ng/mL; (All other analytes; MinDC +2SD); sensitivity: 12.48 ng/mL; (Albumin; MinDC +2SD); standard curve range: 0.01-50 ng/mL; (RBP4); standard curve range: 0.02-100 ng/mL; (B2M); standard curve range: 0.02-100 ng/mL; (TFF-3); standard curve range: 12.21-50,000 ng/mL; (Albumin)
• 기술: multiplexing: suitable
• 검출 방법: fluorometric (Luminex xMAP)
• 배송 상태: wet ice

설명

일반 설명

As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers. In pharmaceutical and chemical industries, renal function is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.

MILLIPLEX^® Canine Kidney Toxicity Panel 2 is a 4-plex kit to be used for the simultaneous quantification of any combination of the following analytes in canine urine samples: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
*Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Toxicity

특이성

Cross Reactivty
Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel.

애플리케이션

• Analytes: Albumin, β-2-Microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
• Recommended Sample Type: Canine urine
• Recommended Sample Dilution: 25 μL of 1:500 diluted canine urine for normal subjects; optimal dilutions for disease subjects should be determined
• Assay Run Time: Primary incubation of 2 hours, followed by a secondary incubation of 1 hour, both at room temperature (20-25°C)
• Research Category: Toxicology

특징 및 장점

Design your multiplex kit by choosing available analytes within this panel.

성분

Canine Kidney Toxicity Panel 2 Standard, 1 vial, lyophilized

Canine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilized

Canine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilized

Assay Buffer, 2 bottles, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Canine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

기타 정보

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analyte sensitivities.

법적 정보

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

안전성 정보

• 픽토그램: GHS06,GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302 + H332,H311,H317,H318,H373,H411
• 예방조치 성명서: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• 표적 기관: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects