ABN1388

Anti-MUL1

from rabbit, purified by affinity chromatography

• 동의어(들): Mitochondrial ubiquitin ligase activator of NFKB 1, E3 SUMO-protein ligase MUL1, E3 ubiquitin-protein ligase MUL1, Growth inhibition and death E3 ligase, Mitochondrial-anchored protein ligase, MAPL, Putative NF-kappa-B-activating protein 266, RING finger
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): rhesus macaque (based on 100% sequence homology)
• 포장: antibody small pack of 25 μg
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_078820
• UniProt 수납 번호: Q969V5
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MUL1(79594)

설명

일반 설명

Mitochondrial ubiquitin ligase activator of NFKB 1 (UniProt: Q969V5; also known as E3 SUMO-protein ligase MUL1, E3 ubiquitin-protein ligase MUL1, Growth inhibition and death E3 ligase, Mitochondrial-anchored protein ligase, MAPL, MAPL, Putative NF-kappa-B-activating protein 266, RING finger protein 218) is encoded by the MUL1 (also known as C1orf166, GIDE, MAPL, MULAN, RNF218) gene (GeneID: 79594) in human. MUL1 is a multi-pass membrane protein located on the outer mitochondrial membrane. It plays a role in the control of mitochondrial morphology and also promotes mitochondrial fragmentation and influences mitochondrial localization. MUL1 displays a weak E3 ubiquitin-protein ligase activity. It contains a RING-type zinc finger domain (aa 302-340), which is required for its E3 ligase activity. MUL1 can ubiquitinate Akt1 preferentially at ′Lys-284′ involving ′Lys-48′-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. When over-expressed, MUL1 is reported to inhibit cell growth by activating JNK through MAP3K7/TAK1 and inducing caspase-dependent apoptosis. MUL1 is ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy.

특이성

This rabbit polyclonal antibody detects Mitochondrial ubiquitin ligase activator of NFKB 1 in Human skeletal muscle tissue.

면역원

KLH-conjugated linenar peptide corresponding to 12 amino acids from the C-terminal region of human Mitochondrial ubiquitin ligase activator of NFKB 1 (MUL1).

애플리케이션

Anti-MUL1, Cat. No. ABN1388, is a highly specific rabbit polyclonal antibody that targets Mitochondrial ubiquitin ligase activator of NFKB 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution of this antibody detected MUL1 in human cerebellum, human skeletal muscle myocytes, and human cerebral cortex.

Research Category
Neuroscience

품질

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MUL1 in 10 µg of human skeletal muscle tissue lysate.

표적 설명

~39 kDa observed; 39.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1