ABE70-I

Anti-BAF180/PBRM1

• 동의어(들): Protein polybromo-1;hPB1;BRG1-associated factor 180;Polybromo-1D
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 분자량: calculated mol wt 192.95 kDa; observed mol wt ~200 kDa
• 정제법: using Protein A
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): nonhuman primates
• 포장: antibody small pack of 100 μg
• 기술: western blot: suitable
• 동형: IgG
• 에피토프 서열: C-terminal half
• 단백질 ID 수납 번호: NP_060783.3?
• UniProt 수납 번호: Q86U86
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PBRM1(55193)

설명

일반 설명

Protein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a widely expressed, bromodomain containing nuclear protein that is an essential component of the SWI/SNF-B (PBAF) chromatin remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling. It contains six bromodomains, two bromo-adjacent homology (BAH) domains (aa 956-1074 and 1156-1272), and one HMG-box (aa 1379-1447). The bromodomains recognize and bind to acetylated lysines and BAH domains mediate protein-protein interaction. Its HMG-box domain binds to DNA. BAF180 acts as a negative regulator of cell proliferation. It plays a role in DNA repair mechanisms and acts as a tumor suppressor. It is also involved in silencing transcription near double-stranded DNA breaks (DSB). The BAH domains of BAF180 are shown to be essential for ubiquitination of PCNA. BAF180 depleted cells can not produce proper levels of ubiquitinated PCNA that leads to the inability to replicate past DNA lesions. Mutations in PBRM1 gene have been linked to high incidence of renal cell carcinoma. Nine isoforms of BAF180 have been described that are produced by alternative splicing. (Ref.: Hopson, S., and Thompson, MJ. (2017). ACS Chem. Biol. 12(10); 2482-2490; Varela, I., et al. (2011). Nature. 469(7331); 539-542).

특이성

This rabbit polyclonal antibody specifically detects Protein polybromo-1 (BAF180). It targets an epitope within the C-terminal half.

면역원

GST-tagged recombinant fragment corresponding to 740 amino acids from the C-terminal half of human BAF180

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protein polybromo-1 (BAF180) in Jurkat cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

물리적 형태

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable