추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human
기술
ChIP: suitable (ChIP-seq)
immunofluorescence: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
mouse ... Lhx2(16870)
일반 설명
LIM/homeobox protein Lhx2 (UniProt Q9Z0S2; also known as Homeobox protein LH-2, LIM homeo box protein 2, Lim2) is encoded by the Lhx2 gene (Gene ID 16870) in murine species. Lhx2 is a transcription factor that plays an important role in controlling the behavior of progenitor cells involved in the development of the skin hair cycling, eye, forebrain, limb, hematopoietic, olfactory, and pituitary systems. Lhx2 is shown to regulate a significant number of hair follicle stem cell (HF-SC) signature genes, as well as cytoskeletal and adhesion molecules within the niche where HF-SCs reside as a single layer of polarized, quiescent epithelial cells. Lhx2 deficiency leads to baldness, because the niche can no longer anchor its hair and maintain the proper behavior of its SCs as a result of greatly perturbed HF-SCs polarization and niche architecture.
면역원
Recombinant protein corresponding to mouse LHX2.
애플리케이션
Immunofluorescence Analysis: A representative lot detected Lhx2 immunoreactivity by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed backskin cryosections from wild-type, but not Lhx2 knockout, mice at varies developmental stages, including E16.5, P0, P30, and the second telogen (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions by a genome-wide ChIP-seq analysis using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Immunofluorescence Analysis: A representative lot (1:1,000 dilution) detected Lhx2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University ).
Chromatin Immunoprecipitation Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions by a genome-wide ChIP-seq analysis using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Immunofluorescence Analysis: A representative lot (1:1,000 dilution) detected Lhx2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University ).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-LHX2 Antibody is validated for use in Western Blotting, Immunofluorescence, ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of LHX2.
품질
Evaluated by Western Blotting in Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected doxycycline-induced LHX2 expression in 10 µg of Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected doxycycline-induced LHX2 expression in 10 µg of Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.
표적 설명
~48 kDa detected. Target band size appears larger than the calculated molecular weight of 44.4 kDa due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.
물리적 형태
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Alicia R Folgueras et al.
Cell stem cell, 13(3), 314-327 (2013-09-10)
In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue
Mazal Cohen-Gulkar et al.
PLoS biology, 21(1), e3001924-e3001924 (2023-01-18)
Tissue-specific transcription factors (TFs) control the transcriptome through an association with noncoding regulatory regions (cistromes). Identifying the combination of TFs that dictate specific cell fate, their specific cistromes and examining their involvement in complex human traits remain a major challenge.
Anna Welle et al.
Glia, 69(9), 2160-2177 (2021-05-25)
Astrocytes from the cerebral cortex (CTX) and cerebellum (CB) share basic molecular programs, but also form distinct spatial and functional subtypes. The regulatory epigenetic layers controlling such regional diversity have not been comprehensively investigated so far. Here, we present an
Yuting Wang et al.
Cell research, 30(5), 408-420 (2020-04-03)
Social hierarchies emerged during evolution, and social rank influences behavior and health of individuals. However, the evolutionary mechanisms of social hierarchy are still unknown in amniotes. Here we developed a new method and performed a genome-wide screening for identifying regions
Mariah L Hoye et al.
eLife, 11 (2022-06-29)
Mutations in the RNA helicase, DDX3X, are a leading cause of Intellectual Disability and present as DDX3X syndrome, a neurodevelopmental disorder associated with cortical malformations and autism. Yet, the cellular and molecular mechanisms by which DDX3X controls cortical development are
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