AB10022

Anti-Histone H2A.X Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Histone H2A.X, Histone H2AX, H2a/x, Histone H2A.X, H2AFX
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human, rat, bovine
• 제조업체/상표: Chemicon^®; Upstate^®
• 기술: flow cytometry: suitable; western blot: suitable
• NCBI 수납 번호: NP_002096
• UniProt 수납 번호: P16104
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: bovine ... H2Ax(531733) ; human ... H2AX(3014) ; mouse ... H2Ax(15270) ; rat ... H2Ax(500987)

설명

일반 설명

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2A.X is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition, this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins at a damaged site.

면역원

Epitope: a.a. 121-139

KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.X.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-Histone H2A.X Antibody, Human (Rabbit Polyclonal Antibody) validated in WB to detect Histone H2A.X also known as H2AFX.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 500 ng of recombinant Histone H2A.X.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of Rat2 acid extract, BALB 3T3/A31 acid extract, and calf thymus histone extract tissue lysate.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Histone H2A.X in 1X10E6 HeLa cells.

품질

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of HeLa acid extract.

표적 설명

~16 kDa observed

물리적 형태

Peptide affinity chromatography

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable