1.50671

ZIC-HILIC^™ (5µm,200Å) 250 x 21.2 mm

stainless steel column, suitable for HPLC, SeQuant^®

• UNSPSC 코드: 41115709

속성

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L114
• 제품 라인: SeQuant^®
• 특징: endcapped: no
• 제조업체/상표: SeQuant^®
• 파라미터: 400 bar pressure; 70 °C max. temp.
• 기술: HPLC: suitable; LC/MS: suitable (Prep LC); mass spectrometry (MS): suitable (High)
• 컬럼 길이 × I.D.: 25 cm × 21.2 mm
• 기질: fully porous particle
• 기질 활성군: ZIC-HILIC bonding phase
• 입자 크기: 5 μm
• 공극 크기: 200 Å pore size
• 작동 pH: 2-7.5
• 분리 기술: hydrophilic interaction (HILIC)
• 저장 온도: no temp limit

설명

일반 설명

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 21.2 mm ID is the best choice for the semi-preparative separation of polar compounds.Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

분석 메모

Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40

법적 정보

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany