07-449-AF488

Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• 동의어(들): Anti-H3.4, Anti-H3/g, Anti-H3FT, Anti-H3t, Anti-HIST3H3, Histone H3.1t, H3/t, H3t, H3/g, trimethyl-Histone (Lys27), Alexa Fluor^™ 488 Conjugate
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 488
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: rat, human, mouse
• 기술: immunocytochemistry: suitable
• 동형: IgG
• NCBI 수납 번호: NM_003493
• UniProt 수납 번호: Q16695
• 배송 상태: wet ice
• 타겟 번역 후 변형: trimethylation (Lys27 )
• 유전자 정보: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

설명

일반 설명

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

특이성

Broad species cross-reactivity expected.

H3K27me3 specificity of the unconjugated antibody (Cat. No. 07-449) has been confirmed using the Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

면역원

Epitope: H3K27me3

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(Kme3)SAP… in which Kme3 corresponds to trimethyl-lysine at residue 27 of human histone H3.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

The unconjugated antibody (Cat. No. 07-449) is shown to be suitable also for immunohistochemistry, immunocytochemistry, immunoprecipitation, multiplex assay, Western blotting, chromatin immunoprecipitation (ChIP), and ChIP-seq applications.

This Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor^™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.

품질

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in A431 cells.

표적 설명

~16 kDa observed

물리적 형태

Protein A purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2