추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
6HH3-2C5, monoclonal
종 반응성
human
종 반응성(상동성에 의해 예측)
Drosophila (based on 100% sequence homology)
기술
immunocytochemistry: suitable
western blot: suitable
동형
IgG1κ
GenBank 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
dimethylation (Lys9), phosphorylation (pSer10)
유전자 정보
human ... H3F3B(3021)
관련 카테고리
일반 설명
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
특이성
Antibody recognizes dimethyl (Lys9) phospho(Ser10) on Histone H3.
면역원
Epitope: Dimethyl (Lys9) & Phospho (Ser10)
Synthetic linear peptide corresponding to dimethyl (Lys9) and Phospho (Ser10) of Histone H3.
애플리케이션
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5 (mouse monoclonal antibody) validated in WB, ICC to detect Histone H3 (Dimethyl K9 also known as H3K9me2S10P, Histone H3 (di methyl K9 phospho S10).
품질
Evaluated by western blot in untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
표적 설명
~17 kDa
물리적 형태
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
Untreated and Etoposide-treated HeLa acid extracts.
Untreated and Etoposide-treated HeLa acid extracts.
법적 정보
GenBank is a registered trademark of United States Department of Health and Human Services
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Histone H3S10 phosphorylation by the JIL-1 kinase in pericentric heterochromatin and on the fourth chromosome creates a composite H3S10phK9me2 epigenetic mark.
Wang, Chao, et al.
Chromosoma (2014)
Jing Sun et al.
Frontiers in plant science, 12, 814465-814465 (2022-02-05)
Lesion mimic mutants have become an effective material for understanding plant-microbe interactions and the immune mechanism in plants. Although many mechanisms responsible for the lesion mimic phenotype have been clarified in plants, the mechanism by which lesion mimic is regulated
Yeran Li et al.
Development (Cambridge, England), 144(18), 3232-3240 (2017-08-16)
A model has been proposed in which JIL-1 kinase-mediated H3S10 and H2Av phosphorylation is required for transcriptional elongation and heat shock-induced chromatin decondensation. However, here we show that although H3S10 phosphorylation is indeed compromised in the H2Av null mutant, chromatin
Scott B Rothbart et al.
Nature structural & molecular biology, 19(11), 1155-1160 (2012-10-02)
A fundamental challenge in mammalian biology has been the elucidation of mechanisms linking DNA methylation and histone post-translational modifications. Human UHRF1 (ubiquitin-like PHD and RING finger domain-containing 1) has multiple domains that bind chromatin, and it is implicated genetically in
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