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Issues	Reasons	Solutions
Weak/low gel formation	Thiol-reactive groups (polymer) and thiol groups (crosslinker) are not near equimolar concentration	Balance the thiol-reactive groups of polymer with thiol groups of crosslinkers
Excess of peptide with thioglycerol results in few thiol-reactive groups	Balance the thiol groups of peptide and crosslinker with thiol-reactive groups of polymer
Low pH caused by acid in peptide sample	Optimize the buffer pH: For a slow gelling system use a buffer with pH 7.2. For a fast gelling system use a buffer with pH 5.5
Weak network formed because of deteriorated reactive groups	Use fresh aliquots
Fast gelation	pH is too high	Use buffer with pH 5.5 to slow down the gelation
Too slow gelation process	pH is too slow	Use buffer with pH 7.2 to accelerate the gelation
Decrease in cell viability	Lack of adhesion factor in polymer	Add appropriate adhesion peptide or proteins
Acidic environment	Use neutral pH during gel setup
Incomplete dissolution of dextran polymer even after 1 hour incubation with TrueGel3D^TM enzymatic cell recovery solution	High gel strength	Increase the incubation period for 2 hours and increase concentration of TrueGel3D^TM enzymatic cell recovery solution
Reduced TrueGel3D^TM enzymatic cell recovery solution activity	Use fresh aliquot of enzyme

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