LC-MS/MS Analysis of Melamine Extracted from Milk on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] 10 mM ammonium formate in 10:90 (v/v) water:acetonitrile; [B] 10 mM ammonium formate in 30:70 (v/v) water:acetonitrile

gradient

held at 0% B for 5 min; 0 to 100% B in 0.1 min; held at 100% B for 4.9 min; 100 to 0% B in 0.1 min; held at 0% B for 4.9 min

flow rate

0.2 mL/min

column temp.

30 °C

detector

ABI 3200 Q-Trap

injection

2 μL

sample

SPE extract from spiked milk