HPLC Analysis of Norphenylephrine Enantiomers on Astec^® CYCLOBOND^™ I 2000 AC (pH 5.0)

column

CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)

mobile phase

[A] methanol; [B] 20 mM ammonium acetate, pH 5.0; (5:95, A:B)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

norphenylephrine, 1 mg/mL in acetonitrile:water (50:50)