HPLC Analysis of Jacobsen Catalyst Enantiomers on Astec^® CYCLOBOND I 2000 HP-RSP (TEA mobile phase)

column

CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] acetonitrile; [B] methanol; [C] acetic acid; [D] triethylamine; (95:5:0.2:0.1, A:B:C:D)

column temp.

25 °C

flow rate

1.5 mL/min

detector

UV, 254 nm

injection

2 μL

sample

5 mg/mL in methanol