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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Methanol, analytical standard
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N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
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Formaldehyde solution, tested according to Ph. Eur.
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Acetone, analytical standard
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Sodium chloride, tested according to Ph. Eur.
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Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
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Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
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Chloroform, analytical standard
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Diethyl ether, analytical standard
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Sodium chloride solution, 0.85%
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Methanol, anhydrous, 99.8%
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Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Tetrahydrofuran, suitable for HPLC, ≥99.9%, inhibitor-free
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1-Octanol, natural, ≥98%, FCC
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1-Octanol, anhydrous, ≥99%
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1-Octanol, ≥98%, FCC, FG
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Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
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Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
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Piperidine, ReagentPlus®, 99%
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Triphenyl phosphite, 97%
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Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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N,N-Dimethylformamide, anhydrous, 99.8%
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Diethyl ether
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Diethylene glycol, ReagentPlus®, 99%