Skip to Content
Merck
  • A fluorescence "turn-on" chemodosimeter for Cu2+ in aqueous solution based on the ion promoted oxidation.

A fluorescence "turn-on" chemodosimeter for Cu2+ in aqueous solution based on the ion promoted oxidation.

Dalton transactions (Cambridge, England : 2003) (2012-02-24)
Junbo Li, Yang Zeng, Qihui Hu, Xianglin Yu, Jia Guo, Zhiquan Pan
PMID22358460
ABSTRACT

We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM, pH = 7.4) buffer containing 50% (v/v) water-CH(3)CN solution.

MATERIALS
Product Number
Brand
Product Description
H7523
Sigma-Aldrich
HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)
H7006
Sigma-Aldrich
HEPES sodium salt, ≥99.0% (titration)
H6147
Sigma-Aldrich
HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
H3375
Sigma-Aldrich
HEPES, ≥99.5% (titration)
H3662
Sigma-Aldrich
HEPES sodium salt solution, BioReagent, 1M, suitable for cell culture
H4034
Sigma-Aldrich
HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
54457
Sigma-Aldrich
HEPES, BioUltra, for molecular biology, ≥99.5% (T)
H3784
Sigma-Aldrich
HEPES sodium salt, BioPerformance Certified, suitable for cell culture, ≥99.0%
RDD035
Sigma-Aldrich
HEPES sodium salt, ≥99.5% (titration), free-flowing, Redi-Dri™
RES6003H-B7
SAFC
HEPES
RES6007H-A7
SAFC
HEPES sodium salt
H3537
Sigma-Aldrich
HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
83264
Sigma-Aldrich
HEPES buffer solution, 1 M in H2O
H0887
Sigma-Aldrich
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture