- Immunofluorescence stimulated emission depletion microscopy.
Immunofluorescence stimulated emission depletion microscopy.
Nature biotechnology (2003-10-21)
Marcus Dyba, Stefan Jakobs, Stefan W Hell
PMID14566345
ABSTRACT
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Abberior® FLIP 565, maleimide, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
Sigma-Aldrich
Abberior® STAR 440SXP, maleimide, for long Stokes STED and 2-color STED application