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G005717

HPLC Analysis of Phenolics from Rosemary on Ascentis® Express C8

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 μm particles (53848-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient: 15 to 100% B in 8.5 min
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: UV, 214 nm
injection: 2 μL
sample: 100 mg/L in 75 μM EDTA, 1.5 mM ascorbate, 15% acetonitrile

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express C8 for the efficient separation of phenolics from rosemary.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Rosmarinic acid
    Carnosol
    Carnosic acid

analytical column

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standard

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