SCP0084

Caspase 3 Substrate

≥95% (HPLC), lyophilized

• Empirical Formula (Hill Notation): C₃₁H₃₉N₅O₁₂
• Molecular Weight: 673.67
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• product name: Caspase 3 Substrate,
• Assay: ≥95% (HPLC)
• form: lyophilized
• composition: Peptide Content, ≥80%
• storage condition: protect from light
• storage temp.: −20°C

Description

Amino Acid Sequence

Ac-Asp-Glu-Val-Asp-4M2NA

General description

Caspase 3 belongs to the family of cysteinyl proteases, which regulate apoptosis. It is encoded by CASP-3 gene. Caspase 3 is important for normal brain development. Caspase 3 is involved in growth stimulation of surviving tumor cells. It is associated with neuronal apoptosis in Alzheimer′s disease.

Application

Caspase 3 Substrate has been used to determine caspase activity in esophageal squamous cell carcinoma EC109 cell line.

Caspase 3, a cysteine protease activator of caspase 6 and caspase 7, metabolizes amyloid-β 4A precursor protein. Caspase 3 may be detected using a variety of chromogenic and fluorogenic peptide substrates built around the DxxD (asp-x-x-asp) sequences DMQD and DEVD such as Ac-DMQD-pNa (Acetyl-Asp-Met-Gln-Asp-p- nitroanalide), chromogenic; Ac-DMQD-AMC (Acetyl-Asp-Met-Gln-Asp- amino-4-methylcoumarin), fluorogenic and Ac-DEVD-4M2Na.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable