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GE28-9909-45

Sigma-Aldrich

Superdex® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

Synonym(s):

Size-exclusion chromatography column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superdex®

mol wt

10000-600000 Da (globular proteins)

manufacturer/tradename

Cytiva 28-9909-45

packaging

1 ea of

parameter

<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume

bed volume

~3 mL

column L × I.D.

15 cm × 5 mm

matrix

agarose dextran composite
polymeric

matrix active group

phenyl phase

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

Related Categories

General description

Superdex 200 is a column for rapid purity check and screening by high resolution gel filtration for proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
Well suited for rapid purity checks and quick screenings of experimental conditions.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocols

Refolding using ion exchange chromatography and analysis of refolding

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Solubilization of Membrane Proteins

This page shows how to solubilize membrane proteins with products from GE Healthcare.

Related Content

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service