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C-24305

Sigma-Aldrich

Melanocyte Growth Medium M2

Ready-to-use kit including phenol red-free Basal Medium and SupplementMix, 500 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

form

aqueous solution

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Melanocyte Growth Medium M2 (Ready-to-use), phenol red-free

Application

Melanocyte Growth Medium M2 is a serum-free and PMA (Phorbol Myristate Acetate)-free Medium that was developed for the optimized in vitro cultivation of human melanocytes. It is also suitable for human pigment epithelial cells, neuronal cells, and melanoma cells. The medium is optimized for primary human cells, but can also be used for equine, porcine, mouse, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin, cultured in M2 MediumNormal Human Epidermal Melanocytes (NHEM), adult donor, cultured in M2 Medium

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium (Ready-to-use): Includes phenol red-free Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial

Quality

All lots of PromoCell Melanocyte Growth Medium M2 are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary Human Melanocytes and Growth Media

Primary Human Epidermal Melanocytes and growth media optimized for in vitro cultivation of human melanocytes, human pigment epithelial cells, melanoma cells, and neuronal cells. Protocols for handling, cell passaging, media, and product use.

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