M6880

Malachite Green oxalate salt

Technical grade

• Synonym(s): N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4
• Empirical Formula (Hill Notation): C₂₃H₂₅N₂ · C₂HO₄ · 0.5C₂H₂O₄
• CAS Number: 2437-29-8
• Molecular Weight: 463.50
• Colour Index Number: 42000
• Beilstein: 3644550
• EC Number: 219-441-7
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897123
• NACRES: NA.47

Properties

• grade: technical grade
• Quality Level: 200
• form: powder, crystals or chunks
• color: metallic green
• solubility: water: 1 mg/mL
• λmax: 616-620 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O
• InChI: 1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1
• InChI key: REZFCYOVFCJTDG-UHFFFAOYSA-M

Description

General description

The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660nm.

Application

Malachite Green oxalate salt has been used in biliary phospholipid analysis.

Malachite Green oxalate salt has been used:

• in the preparation of malachite green-ammonium molybdate reagent, as a stop solution for starch synthase activity assay
• to terminate the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) inhibition assay using phosphatidylinositol (3,4,5) triphosphate (PI(3,4,5)P3) as substrate
• to determine trypanothione synthetase activity to measure inorganic phosphate release from adenosine triphosphate (ATP) during catalysis

Biochem/physiol Actions

Malachite green is used in the treatment of fungal and parasitic infections in fish.

Safety Information

• Pictograms: GHS05,GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 - H318 - H361fd - H410
• Precautionary Statements: P202 - P264 - P273 - P280 - P301 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable